Human Immune Monitoring Center

ATTENTION! If your fund number is missing from your lab, go to the "Membership Requests & Funds" tab, scroll down to the "Request access to additional funds" field, and enter the Cloud Number. If the Cloud Number doesn't appear, please email the Sinai IT Team (Nilesh.Ainchwar@mountsinai.org, Lane.Blumberg@mountsinai.org, Sandeep.KumarVerma@mountsinai.org, Sugandha.Miglani@mountsinai.org, Ayan.Saha@mountsinai.org, Vikas.Telukunta@mountsinai.org, iLab@mssm.edu) to request that the Cloud Number be imported to iLab. If your Cloud Number can't be imported, you'll be referred to SPF@mountsinai.org to get your Cloud Number renewed/extended.
If the Cloud Number is in the lab but you can't make a reservation or purchase, please check with the PI and/or Lab Finance Manager, as they can grant you access to a fund number in the lab. If you can access the Cloud Number but still can't reserve equipment, check with the Core and ensure that training isn't needed to make a reservation. If it's not a training requirement, please email ilab-support@agilent.com for technical support.

Note: iLab pulls Cloud (Fund) Number details from the COA Website (http://coa.mountsinai.org), and if the expiration date shows as expired, iLab purges it from the system.

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Welcome to the HIMC - Human Immune Monitoring Center

Quick Menu:

• Overview of HIMC
• Getting Started
• Location and Hours of Operation
• Links & Resources
• Leadership & Staff Contact Information
• Publication Acknowledgement

 Overview of Services

The mission of the Human Immune Monitoring Center (HIMC) is to provide a comprehensive assessment of the immune status in healthy volunteers and in patients.

HIMC is dedicated to:

1. To assist investigators with the design of immune monitoring plan for clinical studies and clinical trials.
2. To assist investigators with the preparation of grant applications.
3. To provide state of the art immune monitoring assays including extensive cellular and genomic profiling and functional assays.
4. To develop novel immune monitoring strategies to assess immune status in patients
5. To assist with data analysis and biomarker discovery.

Human Immune Monitoring Core Facility Equipment

1. CyTOF2, Mass Cytometer simultaneously resolves multiple parameters with minimal signal overlap, providing unprecedented insights into the functional and phenotypic complexity of biological systems at the single cell level
2. LSRFortessa BD special order analytical flow cytometer (5 lasers) with high throughput capability (96 well format) to support large clinical study analysis
3. Luminex 200 System capable of multiplexing up to 41 analytes simultaneously in a single micro titer plate well
4. QiaSymphony machine for the high throughput (96 samples) automatic nucleic acid isolation
5. RoboSep for magnetic bead based cell separation/isolation
6. QiaCube machine for automated nucleic acid isolation
7. Cryovials shipper in liquid N₂ with inner temperature sensor/monitor
8. Sample bar coding facility for management/tracking via Freezerwork server
9. Nanodrop 2000 Spectrophotometer (ABI), shared equipment
10. ELISPOT reader, shared equipment
11. Coulter Ac-T 5diff Hematology Analyzer, shared equipment
12. BioTek Synergy Neo HTS Multi-Mode Reader with top dual PMT filter optics with integrated BioStack automated stacker, shared equipment
13. BioTek 405™ Touch Microplate Washer with 96-tube Dual-Action™ manifold and integrated BioStack automated stacker, shared equipment
14. Two Tissue culture rooms dedicated for patient sample processing and to perform immune function assays, 2 laminar hoods, 4 tissue culture incubators, centrifuges (two Eppendorf 5425R and 5810R), microscope, water bath
15. Infrastructure dedicated for Microbiome studies from the sample processing to the library prep.
16. Infrastructure for bio-banking including sample collection, processing and storage at dedicated -20^oC, -80^oC and two mobile Liquid nitrogen tanks with temperature monitors

Getting Started

• Register for an account if you're a first time user.

• Log in with your iLab credentials in the upper right corner and go to the Request Services tab to submit a service request or to the Studies tab to submit a new study or initiate work off of an existing study.

• Visit the Customer Help Manual for further guidance using iLab's Core Facility Services.  

Location and Hours of Operation

Contacts