Metabolomics Core

ATTENTION! If your fund number is missing from your lab, go to the "Membership Requests & Funds" tab, scroll down to the "Request access to additional funds" field, and enter the Cloud Number. If the Cloud Number doesn't appear, please email the Sinai IT Team (Nilesh.Ainchwar@mountsinai.org, Lane.Blumberg@mountsinai.org, Sandeep.KumarVerma@mountsinai.org, Sugandha.Miglani@mountsinai.org, Ayan.Saha@mountsinai.org, Vikas.Telukunta@mountsinai.org, iLab@mssm.edu) to request that the Cloud Number be imported to iLab. If your Cloud Number can't be imported, you'll be referred to SPF@mountsinai.org to get your Cloud Number renewed/extended. If the Cloud Number is in the lab but you can't make a reservation or purchase, please check with the PI and/or Lab Finance Manager, as they can grant you access to a fund number in the lab. If you can access the Cloud Number but still can't reserve equipment, check with the Core and ensure that training isn't needed to make a reservation. If it's not a training requirement, please email ilab-support@agilent.com for technical support.

Note: iLab pulls Cloud (Fund) Number details from the COA Website (http://coa.mountsinai.org), and if the expiration date shows as expired, iLab purges it from the system.

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Metabolomics Core

Overview of Services

The Metabolomics Core at Icahn School of Medicine at Mount Sinai (ISMMS) is a newly established facility located in the Mount Sinai West Campus, and is a part of Center for Discovery and Innovation. Our mission is to support ISMMS investigators in their research in the study of metabolism by providing state-of-the-art mass-spectrometry based metabolomics assays. Our services are also available to investigators from other academic institutions and commercial enterprises.

Our facility offers several targeted and untargeted GC-MS and LC-MS based analyses that are validated against a wide range of sample types including body fluids (plasma, serum, saliva, urine), cells, and various tissue types from human subjects and research animals. We are also experienced in performing stable isotope tracing studies using 13C, 15N, and 2H-labeled substrates. We support hypothesis-generating untargeted and hypothesis-driven targeted metabolomics studies from the experimental design stage through data acquisition, data analysis, and interpretation.

Services:

• Relative quantification of amino acid and TCA cycle by GC-MS
• Stable isotope tracing of 13C to TCA cycle by GC-MS
• Polar metabolomics profiling (220 compounds panel) by Agilent QqQ LC-MS
• Targeted metabolomics profiling (exploratory panel) by Thermo Scientific IQ-X LC-MS
• Untargeted metabolomics profiling by Thermo Scientific IQ-X LC-MS
• Stable isotope tracing of 13C, 15N or 2H by Thermo Scientific IQ-X LC-MS

Equipment and technology:

• Agilent 8890/5977C GC-MS
• Agilent 6495D QqQ LC-MS/MS
• Thermo Scientific Orbitrap IQ-X Tribrid LC-MS
• Coy Lab O2 Control In Vitro glove box
• SP Genevac Centrifugal Sample Evaporator
• Omni Inc. Bead Ruptor 12 Tissue Homogenizer

Location and hours of operation

Links and Resources

1. ISMMS Metabolomics CoRE
2. Human Metabolome Database
3. Kyoto Encyclopedia of Genes and Genomes (KEGG) Compound Database
4. Web-based platform for metabolomics data analysis and interpretation

Contacts